How Does An Elisa Testing Work?

The Enzyme-Linked Immunosorbent Assay (ELISA) is a technique used to detect antibodies or infectious agents in a sample. Antibodies are made in response to infection and so antibody ELISA testing can indicate whether or not an animal has been in contact with a certain virus.

ELISA antigen can determine whether an animal is infected with the virus through direct detection. There are many companies available that provide the Human APP / Amyloid Precursor Protein ELISA Kit PicoKine online. You can easily get the ELISA testing kits from various online sources. ELISA results may be interpreted quantitatively, qualitatively or semi-quantitatively.

With antibody ELISA, the antigen attaches to the plastic surface, the sample is added and all antibodies to the disease we tested bind to the antigen.

A second marker antibody is then added and a positive reaction is detected by changing the color of the marker when a suitable substrate is added. If the sample does not contain antibodies, the second antibody will not adhere and no color change will occur.

In antigen ELISA, the antibodies stick to the plastic surface, the sample is added, and if antigens from the virus we are testing are present, they will stick to the antibodies. The test is then performed in the same way as an antibody ELISA.